Ensuring Reliable Cannabis Analysis
Our sister publication, LCGC, covered a presentation from Zacariah Hildenbrand, PhD, in the Analytical Track of the Cannabis Science Conference Fall 2023.
Zacariah Hildenbrand, PhD, served as the chair of the Cultivation Track at the Cannabis Science Conference Fall 2023 and presented two talks in the Analytical Track. Below is coverage of his presentation titled "Getting the Most Out of Analytical Testing," written by Aaron Acevedo.
During a session at the Cannabis Science Conference on September 21, Zacariah Hildenbrand, PhD, a research professor in the Department of Chemistry and Biochemistry at the University of Texas, El Paso gave an overview of various analytical methods that are useful in cannabis analysis. "Cannabis is a versatile plant, with many different compounds in its makeup," Hildenbrand said. Enzymes such as cannabigerolic acid (CBGA), tetrahydrocannabinolic acid (THCA), and cannabidolic acid (CBDA) can all have different effects. Commercial testing protocols vary state by state, which can make it difficult to characterize different products.
“It's absolutely critical that we do this with a high degree of accuracy,” he said. “If you don't have the dosing dialed in, which is predicated on accurate testing, then someone can really have a horrible experience.”
To properly separate these compounds, complex instrumentation is required, with techniques such as high-performance liquid chromatography–ultraviolet (HPLC-UV) and headspace gas chromatography–flame ionization detection (GC-FID). Inductively coupled plasma mass spectrometry (ICP–MS) and inductively coupled plasma–optical emission spectroscopy (ICP–OES) can be useful in finding toxins, and quantitative polymerase chain reaction (qPCR) for detecting DNA of different species. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI–TOF-MS) can be used for characterizing bacteria.
There are also other factors that can complicate cannabis testing, including unreliable laboratories that may run faulty analyses. Because of this, having proper data analysis and troubleshooting procedures is vital to safe and proper cannabis consumption.
Hildenbrand concluded by emphasizing the importance of paying attention to instruments used in commercial testing, specifically regarding calibration and peak shifts.
“If you're not following that key shift and you're not calibrating on a regular basis, you're missing signal, or you put could be mischaracterizing individual species,” he said.
Reference
Hildenbrand, Z. Getting the Most Out of Analytical Testing. In Cannabis Science Conference. Providence, Rhode Island.
Read the original LCGC article by Aaron Acevedo here.
Ep 24, Part III: Data Transparency in Cannabis Testing with Yasha Kahn
December 26th 2024In the final part of this episode, Evan Friedmann and Yasha Kahn discuss the need for a national entity to centralize cannabis data collection, moving from snapshot data to continuous updates. They emphasize the importance of accurate lab data and adverse event tracking, suggesting QR codes on packaging to report issues. Yasha suggests harsher consequences for result manipulation and suggests collaboration between state departments and federal entities to support underfunded regulators. They also discuss the potential benefits of off-the-shelf testing and the importance of stability testing. Finally, Yasha shares his top three reading recommendations for the audience.
Ep 24, Part II: Data Transparency in Cannabis Testing with Yasha Kahn
December 12th 2024Evan Friedmann and Yasha Kahn, co-founder of MCR Labs, discuss the discrepancies between current regulations and data on mycotoxins and pesticides in cannabis products. They highlight the need for updated regulations based on new data, emphasizing the importance of accurate testing and labeling. They also discuss the issue of result manipulation, particularly in THC content, and the need for public health officials to address this. Yasha suggests making testing data public to enhance oversight and suggests a national entity to manage this data for better consistency and public safety.
