Application Note: Recovery & Adsorption of Therapeutic Proteins
We tested the novel filter device Claristep® for the preparation of protein samples containing one of four different target molecules: an anti-malaria vaccine candidate, aviscuminum, interferon alfa-2B, or monoclonal antibody (mAb) P2G12. In comparison to the standard protocols for sample preparation prior to analysis by either reversed-phase or SEC HPLC, the Claristep® filter did not exhibit a significant difference in protein concentration if the target molecules were present with at least 1.0 g L-1. A significantly reduced product concentration was observed in the Claristep® filtered samples compared to the standard protocol for mAb P2G12 if the concentrations were lower. The protein adsorption to the filter material was well described by a Langmuir adsorption isotherm. Additionally, the Claristep® filters facilitated a rapid sample preparation with minimal volume losses.
Determination of Terpenes in Cannabis by GC-FID (using 8300 GC / 8500 GC)
June 14th 2024Hemp and marijuana are becoming more popular because of legalisation in multiple countries. The increase of this market also increases the offer of products and therefore the need to monitor different components. One of these components are cannabinoids or just short potency analysis, which can be found in our portfolio of Cannabis applications (AN091,92,93,130). This application focusses on terpenes analysis.
Cannabis Potency Analysis by HPLC (June 2024)
June 14th 2024CBDV, CBGA, THC-A and CBDA cannot be detected with FID or MS without extra sample preparation due to decarboxylation caused by the heat of the injector. The HPLC is a perfect instrument to measure these components since it does not use heat for the injection. Discover the advantage of this technique being able to analyse acidic cannabinoids without the lengthy sample preparation seen in GC methods.
